摘要
目的:观察CTGF及其上游因子TGF-β1在乙肝病毒相关性肾炎(HBV-GN)肾组织中的表达情况,探讨它们在HBV-GN进展中的作用。方法:采用免疫组化检测87例HBV-GN患者肾活检组织中CTGF和TGF-β1的表达,进行半定量评分,分析其与临床病理指标的相关性。结果:87例中膜性肾病(MN)35例、系膜增生性肾小球肾炎(MsPGN)26例、膜增生性肾小球肾炎(MPGN)11例I、gA肾病(IgAN)9例及其他类型6例。正常肾组织CTGF和TGF-β1呈微弱表达或不表达,HBV-GN肾组织中CTGF和TGF-β1的表达明显高于对照组,不同病理类型中CTGF和TGF-β1的表达差异有统计学意义,CTGF和TGF-β1的表达与肾小管间质病变程度及肾小球的病变程度成正相关。结论:在HBV-GN中CTGF和TGF-β1参与其间质纤维化和疾病的进展。
Objective：To observe the expression of connective tissue growth factor（CTGF） and transforming growth factor-β1（TGF-β1） in hepatitis B virus-associated glomerulo-nephritis（HBV-GN）,and explore their roles in the progress of HBV-GN.Methods：The protein expression of CTGF and TGF-β1 in renal biopsy specimens were determined by immunohistochemistry and tested by semi-quantitative method in 87 patients,who were diagnosed as HBV-GN by renal biopsy.The relation betwwen the expression of CTGF and TGF-β1 and clinicopathologic data was analyzed.Results：Among 87 cases,membranous nephropathy was found in 35 cases,mesangial proliferative nephritis in 26 cases,membranoproliferative nephritis in 11cases,IgA nephropathy in 9 and other pathologic type in 6 cases.The expression of CTGF TGF-β1 was no or faintly found.The expression of CTGF and TGF-β1 in HBV-GN patients＇ renal biopsy specimens was significantly higher than normal kidneys,and significantly different among different pathological types of HBV-GN.Their expression intensity was positive correlated to the lesion degree of renal tubulointerstitium and glomerulus.Conclusion：CTGF and TGF-β1 are important fibrogenic factors in HBV-GN.
出处
《中国中西医结合肾病杂志》
2010年第9期770-773,851,共5页
Chinese Journal of Integrated Traditional and Western Nephrology
基金
福建省科技计划重点项目(No.2007Y0030)
