塞来昔布对骨肉瘤MG-63细胞迁移和侵袭能力的影响

Effects of Celecoxib on Migration and Invasion of Osteosarcoma MG-63 Cell Lines

目的研究选择性COX-2抑制剂塞来昔布对骨肉瘤MG-63细胞迁移和侵袭能力的影响,并初步探讨其相关机制。方法将培养的MG-63细胞分为5组:试验组(分别以25μmol/L、50μmol/L、100μmol/L塞来昔布和50μmol/L塞来昔布+10μg/LPGE2处理)和对照组,采用MTT法、迁移试验、Transwell小室侵袭试验和酶联免疫吸附试验(ELISA)研究不同浓度塞来昔布对骨肉瘤MG-63细胞增殖、迁移能力、侵袭能力以及基质金属酶(MMPs)表达量的影响。结果 MTT法显示塞来昔布作用24h对MG-63细胞增殖无明显影响,作用48h可显著抑制MG-63增殖,且呈现剂量效应关系;迁移试验和侵袭试验表明塞来昔布可抑制MG-63细胞迁移和侵袭,并具有剂量效应关系;酶联免疫吸附试验检测塞来昔布可降低MG-63细胞MMP-2和MMP-9的分泌量,这些抑制效应不能被PGE2完全逆转。结论选择性COX-2抑制剂塞来昔布可抑制骨肉瘤MG-63细胞增殖、迁移和侵袭,这种抑制效应可能是通过减少肿瘤细胞MMP-2和MMP-9的表达来实现的,其机制有部分非COX-2依赖途径存在。

Objective To evaluate the effects of COX-2 selective inhibitor Celecoxib on the migration and invasion of human Osteosarcoma MG-63 cell lines,and study the anticancer mechanisms of Celecoxib.Methods The MG-63 cells were divided into five groups：4 test groups（celecoxib concentrations：25μmol/L,50μmol/L,100μmol/L and Celecoxib 50μmol/L＋ PGE210μg/L）and control group.MTT assay,scratch wound assay and Transwell chamber in vitro invasion assay were used to detect the change of proliferation,migration and invasion of MG-63.The levels of MMP-2 and MMP-9 were evaluated by enzyme-linked immunosorbent assy（ELISA）.Results The proliferation of MG-63 was not inhibited significantly within 24 hours（P0.05）,contrarily,inhibited significantly after 48 hours（P0.05）.Meantime,both migration and invasion of MG-63 cells were depressed.The levels of MMP-2 and MMP-9 were downregulated significantly（P0.05） after 24 hours.All these effects with a dose-dependent manner,could not be reversed completely by PGE2.Conclusion The selective COX-2 inhibitor Celecoxib might inhibit the proliferation,migration and invasion of human Osteosarcoma MG-63 cell lines through downregulating the levels of MMP-2 and MMP-9,which partly was COX-2-independent.