地锦草有效部位抗真菌作用及其机制研究

Active component of Euphorbia humifusa antifungal activity and mechanism

目的初步研究维吾尔药材地锦草有效部位体外抗真菌作用及其机制。方法采用透射电镜观察地锦草有效部位对红色毛癣菌超微结构的影响;高效液相色谱法研究地锦草有效部位对真菌细胞膜麦角甾醇生物合成的影响。结果透射电镜下可见,真菌细胞壁不完整,厚薄不均且局部有缺损,细胞膜轮廓不清,局部有破损,胞内细胞器损伤严重,多呈空泡化,细胞内成分聚集成电子密度较高的团块;高效液相色谱法检测,红色毛癣菌中细胞膜中麦角甾醇含量与其生长对照菌相比,含量降低,差异有显著性,并且有明显的剂量依赖性。结论地锦草有效部位作用于红色毛癣菌后,可使其形态和超微结构发生明显的改变,通过影响真菌细胞膜麦角甾醇的生物合成发挥抗真菌作用。

Aim To explore the mechanism of active component of Euphorbia humifusa.Methods The ultrastructure of Trichophyton rubrum treated with Euphorbia humifusa active component was observed with transmission electron microscopy（TEM）.In addition,the inhibitory effect of Euphorbia humifusa active component on ergosterol biosynthesis in cell membrane was demonstrated by high performance liquid chromatography（HPLC）.Results Analysis of treated cells with TEM showed that the cell wall was disrupted,the thickness of cell wall became uneven and the density of the cell wall decreased.Also,the cell membrane became discontinuous and cellular organelles such as mitochondria and endoplasmic reticulum were damaged.Besides,the cytoplasma was aggregated and some high density electron light areas as well as vacuoles were formed.In addition,Euphorbia humifusa active component inhibited the ergosterol biosynthesis in cell mem-brane of Trichophyton rubrum in a dose-dependent manner demonstrated with HPLC.Conclusion Active component of Euphorbia humifusa has marked antifungal activity,and one mechanism of killing fungi involves inhibiting the ergosterol biosynthesis in fungal cell membrane.