基于炮效关系研究白芥子镇咳药效物质基础

Study on antitussive substance of sinapis alba seeds based on the relationship between drug processing and activity

目的基于炮制方法与药效之间的关系研究白芥子镇咳的药效物质基础。方法采用HPLC比较炒制前后白芥子中化学成分的变化。以体积比为0.25的浓氨水做引咳实验,以实验动物的咳嗽潜伏期、2min内咳嗽次数为评价指标,比较炒制前后白芥子的镇咳作用。分离纯化炒制后含量明显增加的对羟基苯乙腈成分并考察其镇咳作用。结果白芥子炒制后,镇咳作用明显增强,其对羟基苯乙腈含量明显增高。对羟基苯乙腈具有明确镇咳作用,与空白对照组相比,差异具有统计学意义(P<0.05),且中、高剂量对羟基苯乙腈的镇咳作用优于炒白芥子。结论炒制后白芥子镇咳作用增强,炒制法可以增加白芥子中对羟基苯乙腈的含量,对羟基苯乙腈镇咳作用明显,是白芥子镇咳的药效物质。

Aim To study the antitussive substance of sinapis alba seeds through the relationship between processing and the activity.Methods Compare the ingredient content between fresh and roasted sinapis alba seeds by HPLC.The experimental animal cough symptoms were evoked by 0.25 ammonia.Researohers com-pared the antitussive effects between fresh and roasted sinapis alba seeds by cough latent period and cough frequency within 2 minutes.The p-hydroxyphenylacetonitrile that increased markedly after roasting was seperated,and the antitussive effect of p-hydroxyphenylacetonitrile was tested.Results After being roasted,the antitussive effect and the p-hydroxyphenylacetonitrile content of sinapis alba seeds were markedly enhanced.Compared with the negative group,p-hydroxyphenylacetonitrile had stronger antitussive effect,and the difference was statistically significant（P 0.05）.Compared with roasted sinapis alba seeds group,middle and high dose p-hydroxyphenylacetonitrile produced higher antitussive effect.Conclusions The antitussive effect and the level of p-hydroxyphenylacetonitrile in sinapis alba seeds increased after being roasted.The antitussive effects of p-hydroxyphenylacetonitrile is significant,and p-hydroxyphenylacetonitrile is antitussive substance of sinapis alba seeds.