摘要
目的:探讨一种通用的基因组DNA提取方法。方法:采用改良的膜法分别从动植物组织、外周血、细菌、细胞等标本提取基因组DNA,DNA样品经紫外吸收、琼脂糖凝胶电泳、PCR扩增和酶切进行检测。结果:该方法提取的基因组DNA纯度较高,电泳条带清晰,DNA质量能满足下游分子生物学研究的需要。结论:该方法简便快速、适用范围广,是提取基因组DNA的一种有效方法。
Objective:To investigate a universal method for extracting genomic DNA.Method:An improved silica-membrane method was used to extract the genomic DNA from animal and plant tissues,peripheral blood,bacteria and cell.DNA was evaluated through UV spectrophotometer analysis,agarose gel electrophoresis,PCR and restriction enzyme digestion respectively.Result:Higher purity genomic DNA was obtained and it could meet the need of molecular biology manipulation.Conclusion:The method is simple,rapid and can be widely used in different samplesgenomic DNA extraction.
出处
《生物技术》
CAS
CSCD
北大核心
2010年第5期34-36,共3页
Biotechnology
基金
河南省科技攻关项目(0624410041)
河南省自然科学基金项目(2008A310008)资助