IL-28B基因变异与丙型肝炎的易感性及其自然清除的关系

IL-28B genetic variation,susceptibility and spontaneous clearance of hepatitis C virus

目的探讨IL-28B基因变异与丙型肝炎的易感性和丙型肝炎病毒(HCV)自然清除之间的关系。方法利用焦磷酸测序的方法对411例HCV感染者(男性225人,女性186人)及100例健康对照人群进行了IL-28B基因变异位点rs12979860的多态性检测;对HCV感染者进行病毒定量的检测,比较HCV感染人群和健康对照人群,慢性丙型肝炎(CHC)人群和丙型肝炎自愈人群IL-28B基因变异位点的差异以及不同基因型的携带者病毒定量的差异。结果中国健康人群IL-28B基因变异位点rs12979860的最小等位基因频率为6%,与日本人相近,与欧洲人群相差较大。SNP(rs12979860)位点的等位基因频率和基因型频率在HCV感染人群和健康对照人群之间差异无统计学意义(P=0.066;P=0.072),C/C基因型CHC人群病毒定量水平高于C/T基因型,二者之间差异有统计学意义(P=0.02);丙型肝炎自愈人群中C/C基因型和C/T基因型差异无统计学意义(P=0.64)。结论 CHC人群rs12979860 SNP基因型与健康人群表达无明显差别,该基因型(rs12979860)不是HCV的易感基因,其CC基因型并不能预测丙型肝炎发生自愈的病程。

Objective To explore the association between IL-28B genetic variation,susceptibility and spontaneous clear-ance of hepatitis C virus.Methods A total of 411 hepatitis C patients（255 male and 186 female） and 100 healthy con-trols were enrolled in the study.The rs12979860 SNP of IL-28B was identified from each cohort using pyrosequencing technology.HCV-RNA was tested in hepatitis C patients.The IL28B genetic variation was compared between hepatitis C patients and healthy controls,and chronically infected and spontaneously cleared HCV groups to assess the effect of SNP.Results The minor allele frequency（rs12979860） of Chinese population was 6%.There was no genotype and allele fre-quency（rs12979860） difference between hepatitis C patients and healthy controls（P = 0.066,P = 0.072）.The level of HCV-RNA in genotype CC was significantly higher than genotype CT groups（P = 0.02）.There was no significant differ-ence between genotype CC and CT among individuals who spontaneously clear HCV（P = 0.64）.There was no significant difference in SNP genotype（rs12979860） between chronically infected groups and healthy controls.Conclusion The SNP genotype rs12979860 may not be the predisposing gene of hepatitis C and the CC genotype cannot predict the course of spontaneous clearance of hepatitis C.