HBV慢性感染者和非感染者IL-10启动子基因多态性比较

The frequency of Interleukin -10 gene promoter polymorphism in chronic HBV patients

目的探讨IL-10启动子基因多态性对HBV感染者易感性和慢性化的影响。方法选择52例慢性乙型肝炎患者为观察对象,采用限制性长片段多肽分析和直接测序的方法,检测IL-10启动子基因多态性,并与48例健康人群基因分布频率进行比较。结果从等位基因单基因分布频率分析比较,病毒携带者和健康人群组分布频率无显著统计学差异;从基因型分布频率比较两者间无显著差异;从单倍体分析,两组间比较也无明显差异。无论是HBV慢性感染者还是健康人群在-1082位的等位基因A和-819位的等位基因T有较高的分布频率。结论 IL-10启动子基因多态性可能对HBV易感性和慢性化无显著影响。

Objective To study the effect of the polymorphisms of interleukin-10（IL-10） gene promoter on susceptibility and chronicity of HBV.Methods A total of fifty two HBV carriers and 48 healthy controls were included.Three bi allelic polymorphisms in the IL-10 gene promoter were analyzed by polymerase chain reaction in combination with direct sequencing or restriction fragment length polymorphism assay.Results There was no difference in the frequencies of allelics,genotypes and haplotypes between the two groups.The allelics of A（-1082） and T（-819） in IL-10 gene promoter were high in both groups.Conclusion In chronic HBV infection,the polymorphisms of IL-10 gene promoter do not play a significant role in the susceptibility and chronicity of HBV.