染料木素促成骨样细胞增殖作用及其机制研究

Effect and mechanism of genistein on the proliferation of MC3T3-E1 cells

目的观察染料木素(genistein)对成骨样细胞MC3T3-E1细胞增殖及骨形成蛋白-2(BMP-2)、β-连环素(β-catenin)表达的影响。方法培养成骨样细胞MC3T3-E1,加入不同浓度的染料木素(1×10-10、1×10-9、1×10-8、1×10-7、1×10-6和1×10-5mol·L-1),以1×10-9mol·L-1雌激素(β-estradiol,E2)作为阳性对照组,采用MTT比色试验法和碱性磷酸酶(ALP)活性检测法,观察染料木素作用后MC3T3-E1的增殖及分化情况,以Westernblot方法检测成骨样细胞中BMP-2和β-catenin蛋白的表达。结果染料木素可促进MC3T3-E1细胞增殖,染料木素作用后细胞的ALP值也明显升高,呈浓度依赖性;染料木素可增加BMP-2和β-catenin表达,且呈浓度依赖性。结论染料木素促进MC3T3-E1细胞增殖与分化,可通过调节BMP-2和Wnt/β-catenin信号通路而影响成骨样细胞的活性。

Aim To investigate the effect of genstein on the proliferation and differentiation of MC3T3-E1 osteoblast cells as well as the expression of BMP-2 and β-catenin on protein levels.Methods Genistein was administrated to MC3T3-E1 cells at the concentrations of 1×10 -10,1×10-9,1×10-8,1×10-7,1×10-6 and 1×10 -5 mol·L -1 in DMEM.β-estradiol at the concentration of 1×10 -9 mol·L -1 was used as a positive control.And the effect on osteoblast proliferation and differentiation were assessed by MTT and ALP assays;the protein levels of BMP-2 andβ-catenin in MC3T3-E1 were evaluated by Western blot.Results Proliferation of MC3T3-E1 was promoted by different concentrations of genistein;Results of Western blot examination revealed that genistein increased BMP-2 and β-catenin expression in a dose-dependent manner. Conclusions The results indicate that genistein can promote proliferation and differentiation in MC3T3-E1 by increasing BMP-2 expression and enhancing Wnt/βcatenin signal pathway.