喉鳞状细胞癌中PIN1基因扩增及表达异常

PIN1 gene amplification and overexpression in human laryngeal squamous cell carcinoma

目的:研究PIN1基因在喉癌组织中的表达情况。方法:采用差异PCR、核型分析方法检测喉鳞癌及癌旁对照组织中PIN1基因扩增情况;采用RT-PCR检测同批组织中PIN1 mRNA表达情况;采用免疫组织化学、Western Blot方法检测PIN1蛋白表达情况。结果:与癌旁对照组织相比,40例喉癌组织中有9例(9/40,22.5%)存在PIN1基因扩增,27例(27/40,67.5%)PIN1 mRNA过表达,26例(26/40,65%)PIN1蛋白过表达(P<0.05)。40例中17例原代培养成功,可制备中期染色体标本,其中3例(3/17,17.65%)存在19号染色体增加。结论:喉鳞状细胞中PIN1基因拷贝数增加,PIN1 mRNA和蛋白质过表达,可能与喉癌发生发展有关。

OBJECTIVE：To investigate the amplification and overexpression of PIN1 gene in human laryngeal squamous cell carcinoma. METHODS： PIN1 gene amplification（increase of gene copy number） was detected by differential PCR and Karyotype analysis. PIN1 mRNA was evaluated by RT-PCR. Furthermore,immunohistochemistry and Western Blot were employed to detect the PIN1 protein. RESULTS： Compared with nomal control,PIN1 gene amplification rate was 22.5%（9/40）,while 17.65%（3/17） was found to have an increase of chromosome 19; the rate of mRNA overexpression was 67.5%（27/40）. PIN1 protein was obviously upregulated,the ratio was 65%（26/40）（P0.05）. CONCLUSION： PIN1 gene copy increased,with mRNA and protein overexpression in laryngeal carcinoma. This fact may be relevant to laryngeal cancer development.