百草枯对金鱼血清过氧化物酶和酯酶表达的影响

Effect of paraquat on the expressions of peroxidase and esterase in goldfish serum

目的:探讨除草剂百草枯(paraquat)对金鱼(Carassius auratus)血清过氧化物酶(peroxidase,POD)和酯酶(esterase,EST)表达的影响。方法:依据百草枯的24h半数致死剂量设置0.5、1.0、1.5、2.0ml/L共4个浓度组,每个浓度组按0.1ml/g剂量金鱼腹腔注射给药,染毒后断尾取血,采用聚丙烯酰胺方法检测金鱼血清POD和EST的表达,对照组金鱼腹腔注射等量生理盐水。结果:与对照组相比,百草枯4个浓度组对金鱼血清POD和EST的活性均有显著影响,在低于半数致死浓度(0.5、1.0ml/L)时POD酶活性增强,EST酶活性被抑制,表现为应急性;高于半数致死浓度(1.5、2.0ml/L)时POD酶活性先减弱后增强,EST酶活性先增强后减弱,酶活性与染毒浓度存在剂量-效应关系。结论:百草枯对金鱼POD和EST均有影响,且对EST的毒性大于POD。

OBJECTIVE：To explore the effect of herbicide paraquat on the expression of peroxidase（POD） and esterase（EST） in Carassius auratus serum. METHODS： The 0.5,1.0,1.5 and 2.0 ml/L concentrations of paraquat were prepared according its 24 h LC50,Carassius auratus were treated with 0.1 ml/g dose of each concentration. SDS-polyarylamide gel were used to test the expressions of POD and EST. RESULTS： Compared with the control group,the four treated groups showed significant variety. The activities of POD and EST increased or decreased at low concentration,absent or increased at high paraquat concentration,concentration and activity had dose-effect relationship. CONCLUSION： The toxicity of paraquat on POD and EST varied in Carassius auratus.