烯啶虫胺的致突变性试验

Mutagencity test of nitenpyram

目的:研究烯啶虫胺的致突变性,为其安全生产和使用提供参考依据。方法:采用小鼠骨髓嗜多染红细胞微核试验、Ames试验、中国地鼠肺细胞(CHL)染色体畸变试验和小鼠淋巴瘤细胞L5178YTK基因突变试验检测烯啶虫胺的致突变性。结果:烯啶虫胺未引起骨髓嗜多染红细胞微核率的异常增高(P>0.05);在直接作用或代谢活化条件下,鼠伤寒沙门氏菌TA97、TA98、TA100、TA102各剂量组回复突变菌落数均未超过自发回变菌落数的2倍;烯啶虫胺各剂量组的CHL细胞染色体畸变率和L5178Y细胞TK基因突变频率,与阴性对照组比较,其差异均无统计学意义(P>0.05)。结论:在本试验条件下,未发现烯啶虫胺有致突变性。

OBJECTIVE：To study the mutagenicity of nitenpyram,and provide basis for safety in production and application. METHODS： Micronucleus test of polychromatic erythrocytes（PCE） in mice bone marrow,Ames test,chromosomal aberration test of Chinese hamster lung cell （CHL） and TK gene mutation assay of mouse lymphoma cell （L5178Y） were taken to examine the mutagenicity of nitenpyram. RESULTS： Nitenpyram didn＇t cause the increase of micronucleus rates （P0.05）. The number of revertant colonies was no more than twice the spontaneous colonies for each dosage group.Compared with the control group,there was no significant difference for chromosomal aberration rates and mutation frequency of TK gene in each dosage group（P0.05）. CONCLUSION： Mutagenic effects of nitenpyram were not detected under these test condition.