圆齿野鸦椿种子外植体的快繁体系

Rapid propagation system of explants of Eascaphis konlshli seeds

以圆齿野鸦椿的成熟种子为外植体,研究组培繁殖育苗技术,以筛选最适的培养基配方.结果表明:(1)激素种类和含量对种子无菌株系建立的影响不同,BA含量为0.5-2.5 mg.L-1时,种子的成活率随BA含量的升高而下降,而NAA对其影响不大,种子无菌株系建立的最佳培养基为MS+0.5 mg.L-1BA+0.3 mg.L-1NAA+7.5 g.L-1琼脂+20 g.L-1蔗糖;(2)在细胞分裂素含量相同的情况下,芽的增殖系数随着生长素含量的增加呈先增大后减小的趋势,在MS+1.0 mg.L-1BA+0.5 mg.L-1NAA+1.0 mg.L-1IAA+7.5 g.L-1琼脂+20 g.L-1蔗糖培育基上,发芽率达50%,增殖系数为3.70,芽饱满健壮;(3)细胞分裂素和生长素适当的配比是芽增殖的关键,最佳培养基为MS+2.0 mg.L-1BA+0.3 mg.L-1NAA+1.0 mg.L-1IAA+7.5 g.L-1琼脂+20 g.L-1蔗糖,增殖倍数达5.0;(4)一定含量的NAA有利于诱导长根,而IBA则不同,培养基中NAA的含量为0.1 mg.L-1,IBA的含量为0.5 mg.L-1时,生根率达95%,根长为3.51 cm.

This experiment studied the seedling techniques of tissue culture,which used the mature seeds of Eascaphis konlshli Hayata as explants,selecting out the optimum medium formula.（1） Establishment of aseptic strain： the concentration of BA was 0.5-2.5 mg·L-1,the survival rate declined with the rising of concentration,but it was insignificantly influencd by NAA,the most suitable medium was MS＋0.5 mg·L-1 BA＋0.3 mg·L-1 NAA＋7.5 g·L-1 agar＋20 g·L-1 sucrose;（2） Bud induced： under the condition of the same cell mitogen concentration,proliferation coefficient tended to firstly increase and then decrease following the increase of auxin concentration,MS＋1.0 mg·L-1 BA＋0.5 mg·L-1 NAA＋1.0 mg·L-1 IAA＋7.5 g·L-1 agar＋20 g·L-1 sucrose was the most suitable bud inducing medium,the germination percentage was 50%,the proliferation coefficient was 3.70 and the bud was in perfect condition;（3） Shoot proliferation： the key of proliferation was the suitable matching percentage of cytokinin and auxin,MS＋2.0 mg·L-1 BA＋0.3 mg·L-1 NAA＋1.0 mg·L1 IAA＋7.5 g·L-1 agar ＋20 g·L-1 sucrose was the best shoot proliferation medium,and the proliferation multiple would be 5.0;（4） Rooting： the particular concentration of NAA was good for inducing long root while IBA not,using hormone combination（0.1 mg·L-1 NAA＋0.5 mg·L-1 IBA）,the rooting rate would be 95% and the average length would be 3.51 cm.