肌苷酸合成酶系2个基因对苏禽乌骨鸡胸肌肌苷酸含量的遗传效应

Analysis of the genetic effects of two genes involved in de novo purine biosynthesis on IMP content in Suqin silky

采用PCR-SSCP方法检测苏禽乌骨鸡ADSL基因外显子2和GARS-AIRS-GART基因5′侧翼区多态性,分析多态位点不同基因型及聚合基因型与90日龄胸肌肌苷酸(IMP)含量的关联.结果表明,ADSL基因外显子2和GARS-AIRS-GART基因5′侧翼区的多态位点C3484T、C-179T与IMP含量显著相关(P<0.05),TT为2种有利单基因型,其对IMP含量的加性效应分别为0.108和0.067 mg.g-1,显性效应分别为-0.065和-0.136 mg.g-1.2种有利单基因型聚合个体TTTT的IMP含量显著高于其他基因型聚合个体(除TTCC型),且在聚合基因型中2种有利单基因型TT对IMP含量的影响均达到显著水平(P<0.05),但两者间的互作效应并不显著(P>0.05).

This experiment was designed to study the polymorphism sites in exon 2 of ADSL and 5′-flanking sequence of GARS-AIRS-GART on Suqin silky by PCR-SSCP.Association analysis between the polymorphic sites as well as pyramiding genotypes and inosine monophosphate acid（IMP） contents in breast muscles were tested by least square means.The results showed that the polymorphisms C3484T and C-179T had significant association with IMP contents in breast muscles（P0.05）.The TT was an advantageous genotype for two loci.Their additive effects were 0.108 mg·g-1 and 0.067 mg·g-1,respectively.And their dominant effects were-0.065 mg·g-1 and 0.136 mg·g-1,respectively.The pyramiding genotype TTTT had higher IMP contents than those with other pyramiding genotypes.The effects of two advantageous TTs in pyramiding genotype TTTT still had significant effect on IMP contents.However,their interaction effect was not significant（P0.05）.