GATA-4在小鼠睾丸中的表达定位研究

Determination of GATA-4 in the testis of the mouse

目的:观察小鼠睾丸内GATA-4免疫反应产物的特征与分布。方法:选取刚出生、2、4、6周雄性B6SJLF1/J小鼠各6只,取睾丸进行石蜡切片,应用免疫组化ABC法,DAB显色,观察GATA-4在不同时期睾丸中的表达情况。结果:GATA-4免疫反应产物在以上4个阶段小鼠睾丸Sertoli细胞、Leydig细胞均有分布,且4、6周组小鼠睾丸Leydig细胞阳性率比刚出生和2周组小鼠更高(P<0.01);但4、6周组小鼠,除以上两种细胞外,生精细胞中也有分布,6周组小鼠比4周组小鼠阳性细胞率高(P<0.01)。结论:小鼠睾丸内存在GATA-4,为研究GATA-4在睾丸性别决定和分化以及激素调控方面提供了形态学依据。

Objective：To explore the characteristics and distribution of GATA-4 in the testis of male mice. Methods：Paraffin sections were obtained from the testes of 24 male B6SJLF1/J mice,aged 0 day （n = 6）,2 weeks （n = 6）,4 weeks （n = 6） and 6 weeks （n = 6）,and the expressions of GATA-4 in the testis were observed by the immunohistochemical ABC method and DAB visualization at different times. Results：Positive expressions of GATA-4 were found in the Sertoli cells and Leydig cells of all the mice,but significantly higher in the 4-and 6-week-old than in the 0-day and 2-week-old groups （P 0.01）. And they were also observed in the germ cells of the 4-and 6-week-old mice,significantly higher in the latter than in the former （P 0.01）. Conclusion：GATA-4 exists in the testis of male mice,which has provided a morphological base for sex determination and differentiation and hormone regulation in the testis.