乙肝病毒YMDD变异与乙型肝炎病毒大蛋白相关性的探讨

Study on the relations between hepatitis B virus large surface protein （LHBs） and HBV YMDD mutation

目的 通过检测乙型肝炎病毒YMDD变异患者血清中的乙型肝炎病毒大蛋白（Hepatitis B Virus Large Surface Protein,LHBs）,探讨乙型肝炎病毒大蛋白（LHBs）的检测与YMDD变异的关系.方法 选取经过拉米夫定治疗后的患者,进行YMDD变异检测,再对所有患者进行HBVDNA和乙型肝炎病毒大蛋白（LHBs）的检测;YMDD变异和HBV DNA检测采用荧光定量PCR法,LHBs检测采用酶联免疫法（ELISA）.结果 65例YMDD变异患者中,HBV DNA拷贝数对数值与LHBs吸光度（A值）呈良好的相关关系（r=0.961）;75例非变异患者中,HBV DNA拷贝数对数值与LHBs吸光度（A值）呈良好的相关关系（r=0.954）.结论 在YMDD变异患者和非变异患者中LHBs吸光度与HBV DNA拷贝数均具有良好的正相关性,表明患者体内LHBs与病毒复制程度密切相关,而且不受乙肝病毒YMDD变异的影响.

Objective To explore the Relations between hepatitis B virus large surface protein （LHBs） and HBV YMDD mutation, By detecting the hepatitis B virus large protein （LHBs）in Serum of patients with hepatitis B virus YMDD mutation. Methods Selected patients after lamivudine treatment, to HBV YMDD mutation detection,then detected LHBs and HBV DNA in Serum of all patients. Serum HBV-DNA was quantitively detected by using real-time polymerase chain reaction （RT-PCR）, the LHBS was detected by using enzyme linked immunosorbent assay （ELISA）. Results There was positive correlation between the A value of LHBs and HBV DNA level in 65 cases of mutation patients （r=0.961）. There was positive correlation between the A value of LHBs and HBV DNA level in 75 cases of non-mutation patients（r =0.954）. Conclusion There were positive correlation between the A value of LHBs and HBV DNA level in non-mutation patients and mutation patients, showed that LHBs can partially reflect the level of viral replication in patients, but not the impact of hepatitis B virus YMDD mutation.