摘要
目的从黑骨藤(HGT)中分离筛选免疫抗炎活性部位,探讨其抗炎及免疫调节作用,为研制和开发新药提供实验依据。方法应用巴豆油诱发的急性小鼠耳肿胀及二硝基氯苯(DNCB)诱发的小鼠迟发型超敏反应(DTH)作为免疫抗炎活性的评价指标,观察HGT-5A对急性炎症及细胞免疫反应的影响。结果 HGT-5A100～500mg·kg-1灌胃给药对巴豆油诱发的小鼠急性耳肿胀具有明显抑制作用,50～100mg·kg-1灌胃给药对DNCB诱导的DTH反应具有明显抑制作用,并且对DTH模型小鼠脾细胞增殖反应均具有明显抑制作用。结论 HGT-5A具有较明显的抗炎和免疫调节作用。
Objective To find out the fraction with anti-inflammatory and immune modulatory activities form Periploca forrestii Schltr （HGT）,and evaluate its action.Methods Croton oil-induced ear edema was used to assess the anti-acute inflammation effect of HGT-5A.2,4-Dinitrochlorobenzene（DNCB）-induced delayed hypersensitivity（DTH） response was employed to observe the effect on cellular immune response.Results HTG-5A significantly inhibited croton oil-induced ear edema at 100-500 mg·kg-1 （ig） and also surpressed DNCB-induced cellular immune response at 50-100 mg·kg-1 （ig）.The splenocyte proliferation was decreased after incubation with HGT-5A.Conclusion HGT-5A has strong anti-inflammatory and immune regulatory activities.
出处
《医药导报》
CAS
2010年第11期1425-1428,共4页
Herald of Medicine
关键词
黑骨藤
炎症
急性
细胞免疫
Periploca forrestii Schltr
Inflammation
acute
Cellular immune
