参麦注射液对兔关节炎模型关节液透明质酸水平的影响

Effects of Shenmai injections on hyaluronic acid level in the rabbit model of osteoarthritis

目的 从透明质酸（HA）方面研究参麦注射液关节腔注射治疗膝骨关节炎的机理.方法 清洁级新西兰白兔30只,随机分为治疗组、对照组及空白组,每组各10只.将对照组和治疗组采用改良Hulth法造模,空白组及对照组予生理盐水1 ml右后腿膝关节腔注射,治疗组用参麦注射液0.8 ml＋0.2ml 2%利多卡因关节腔注射,1次/周,连续4周.于第1、4周抽取关节液,Elisa检测关节液中HA含量.结果 治疗组与对照组治疗前HA含量均比空白组低（P〈0.01）,差异有显著性.治疗后,治疗组HA含量较前有所增高,对照组升高不明显,治疗组与对照组相比,治疗组HA含量增高显著（P〈0.01）.结论 参麦注射液关节腔注射治疗可能通过提高HA含量来治疗膝骨关节炎.

Objective To explore the mechanism of intraarticular injections of Shenmai in the treatment of osteoarthritis by research on hyaluronic acid （ HA ）. Method 30 male Clean-class New Zealand white rabbits were divided into treatmene group, control group, and blank group. The model of osteoarthritis was established by modified Hulth method in the first two groups. Then the treatment group received an intraarticular injection of Shenmai of 0.8 ml daily for 4 weeks and the control group received 1 ml of normal saline. Synovial fluid was extracted to detect HA level by Elisa on weeks 1 and 4. Results Baseline HA level was significantly lower in the treatment and the control group than in the blank group（ P〈0.01 ）; After therapy, the HA level was markedly higher in the treatment group than in the control group （ P〈 0.01 ）. Conclusions Intraarticular injections of Shenmai can be used to treat osteoarthritis by raising HA level in the synovial fluid.