摘要
目的了解长期低剂量接触2,3,7,8-四氯二苯并-p-二噁英(TCDD)对雄性SD大鼠骨髓细胞微核和精子形态的影响。方法将1月龄SD大鼠随机分为4组,分别为阴性对照组(给予玉米油)和3个TCDD染毒组(日染毒剂量为2、10、50ng/kg)。13周后,另设微核阳性对照组(环磷酰胺,日染毒剂量为60mg/kg,连续3d)观察骨髓嗜多染红细胞微核率和精子畸形率。结果长期低剂量接触TCDD导致大鼠骨髓细胞微核率增加,精子畸形率增高。阴性对照组、2、10、50ng/kg染毒组、阳性对照组微核率分别为9.0‰,13.0‰,21.2‰,24.4‰和87.4‰,10、50ng/kg组微核率均高于阴性对照组(均P<0.01);对照组、2、10、50ng/kg组精子畸形率分别为10.4‰,35.0‰,49.8‰,47.0‰,各染毒组畸形率均高于对照组(均P<0.01),畸形以颈折叠为主。结论长期低剂量接触TCDD对哺乳动物体细胞和雄性生殖细胞具有致突变性,且生殖细胞对TCDD的毒性更敏感。
Objective To study the effects of low dose-long term TCDD exposure on micronuclei（MN） of bone marrow and sperm abnormality in male SD rats.Methods One-month-old rats were divided randomly into 4 groups：a negative control,and three dose groups treated with TCDD daily：2 ng/（kg·d）,10 ng/（kg·d）,50 ng/（kg·d） respectively.Thirteen weeks later,a positive control was set [cyclophosphamide,60 mg/（kg·d）,i.p.,3 days before micronucleus test],one thousand bone marrow polychromatophilic erythroblasts（PCE） and one thousand sperms were counted in each rat and the micronuclei（MN） rate and sperm deformity rates were calculated.Results Low-dose and long-term TCDD exposure increased the MN rate and sperm abnormality rates in rats.The MN rates of the negative control,2 ng/（kg·d）,10 ng/（kg·d）,50 ng/（kg·d） and the positive control groups were 9.0‰,13.0‰,21.2‰,24.4‰ and 87.4‰ respectively.The rates of the two higher-dose groups were significantly higher than the negative control（both P〈0.01）;The sperm abnormity rates of the negative control,2 ng/（kg·d）,10 ng/（kg·d）,50 ng/（kg·d） groups were 10.4‰,35.0‰,49.8‰,47.0‰ respectively.The sperm abnormity rates of each dose group was significantly higher than that of the negative control（all P〈0.01）.The main abnormity type was neck-folding.Conclusion Low-dose and long-term TCDD exposure has obvious mutagenic effects on the somatic and germ cells in mammals,and germ cells in male animals are more sensitive to TCDD.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2010年第9期788-791,共4页
Journal of Environment and Health
基金
广东省自然科学基金资助项目(05301076)
教育部留学回国人员科研启动基金资助项目(2005)
