熊果酸对卵巢癌细胞转移的抑制作用

Effects of UA on metastasis of ovarian carcinoma cell

目的 熊果酸（UA）对人卵巢癌细胞株HO-8910PM黏附、侵袭及趋化运动的影响,并探讨其可能的作用机制.方法 采用MTT法方法检测熊果酸对HO-8910PM细胞黏附能力的影响;Transwell小室进行人工重组基底膜侵袭实验检测熊果酸对HO-8910PM细胞侵袭及运动的影响;采用RT-PCR和Western blot方法检测熊果酸对HO-8910PM细胞中MMP-2 mRNA、MMP-9 mRNA和蛋白的表达的影响.结果 在黏附试验中,不同浓度熊果酸作用细胞24h后,抑制率分别为44.03%、46.62%和55.11%,与对照组比较具有统计学意义（P＜0.05）.不同浓度的熊果酸在体外作用24h后可以显著抑制HO-8910PM细胞的侵袭能力,穿膜细胞数与对照组比较具有统计学意义（P＜0.01）.熊果酸处理后细胞趋化运动能力降低与对照组比较差异有统计学意义（P＜0.05）.不同浓度熊果酸作用24小时后,HO-8910PM细胞MMP-2 mRNA、MMP-9 mRNA表达量明显低于正常对照组（P＜0.05）,Western blot结果显示：HO-8910PM细胞MMP-2、MMP-9蛋白表达量受到明显抑制（P＜0.05）.结论 熊果酸抑制人卵巢癌细胞株HO-8910PM黏附、侵袭和转移的生物学行为,其作用机制可能与熊果酸显著抑制MMP-2、MMP-9表达水平有密切关系.

Objective To observe the effects of Ursolic acid（UA） on adhesion,invasion and migration of HO- 8910PM cell line in vitro, and discuss its possible mechanism. Methods Effect of UA on adhesion of HO-8910PM cells were determined with MTT assay, the effects of UA on invasion and migration of HO- 8910PM cells were evaluated using Transwell chambers attached with polycarbonate fillers and reconstituted base- ment membrane. The exprssion of MMP - 2 mRNA, MMP - 9 mRNA was measured by RT - PCR in HO - 8910PM cells,the protein expression of MMP -2, MMP -9 was analyzed by Western blot. Resuls In the cell - Matrigel adhesion experiment,inhibition rate of U A groups at different concentrations of 10,20,30 μmol/L for 24 h were 44.03% ,46.62% and 55. 11% respectively which were significantly higher than those of control groups （P 〈0.05 ） , after 24 hours, the ability of the different concentrations of UA treated HO- 8910PM cells to invade the reconstituted basement membrane decreased significantly（ P 〈 0.01 ）. UA can also inhibit HO -8910PM cells migration （P 〈 0.05 ）. RT - PCR and Western blot resnhs showed decreased expression of MMP - 2, MMP - 9 in HO- 8910PM cells treated with（10,20,30μmol/L） UA for 24 hours. Conclusion The inhibition of invasion ability of HO -8910PM cells by UA may be attributed to decreases of the expression of MMP- 2 ,MMP- 9.