毛细管区带电泳测定伸筋丹胶囊中士的宁和马钱子碱的含量

Determination of Strychnine and Brucine in Shenjindan Capsule by Capillary Zone Electrophoresis

应用毛细管区带电泳法，对伸筋丹胶囊中的士的宁和马钱子碱成分进行了分离研究。采 用ｐＨ＝６．０的０．２ ｍｏｌ／Ｌ．ｔｒｉｓ－磷酸溶液作为缓冲液；运行电压２０ ｋＶ；检测波长为２６０ｎｍ，被测成 分可得到较好分离。用内标法测定其含量，结果表明，在 ０．０２～０．１５ ｍｇ／ｍＬ范围内，士的宁和马 钱子碱均与内标峰面积之比呈良好线性关系，平均回收率分别为９５．１％（ＲＳＤ＝２．８％）和９６．４％ （ＲＳＤ＝３．０ ％）。

A capillary zone electrophoresis method was established for the determination of strychnine and brucine in Shenjindan capsule, a preparation composed of & different TCM including Semen Strychni and dried earthworm for relieving rigidity of muscles by promoting blood circulation to remove blood stasis. 0. 2 mol/L tris-H3PO4 (pH= 6. 0) was used as running buffer, running voltage was 20 kV, and UV absorbance detection was set at 260 um, This method can give a good separation for the determination of strychnine and brucine. By using internal standard the calibration curve showed good linearity for strychnine and brucine, average recovery was 95. 1% for strychnine and 96. 4% for burcine.