利用SRAP技术分析茭白种质资源遗传多样性

Genetic diversity analysis of water bamboo(Zizania latifolia Turcz.)germplasms in Zhejiang employing SRAP markers

采用简化的SRAP分析方法,用47对SRAP引物对35份茭白材料进行遗传多样性分析,共获得153个扩增位点,其中多态性位点11个,平均多态水平为7.2%,扩增产物的片段大小范围在50~400 bp。利用UPGMA法进行聚类分析,以遗传相似系数0.51为阈值,将35个茭白品系分为2个类群。单双季茭间遗传距离大于各类型内部差异,来源地相同的材料具有更高的遗传相似性。

The genetic diversity analysis among 35 accessions of water bamboo（Zizania latifolia Turcz.） from Zhejiang Province was conducted using 47 pairs of SRAP primers.A total of 153 loci were detected,11 being polymorphic,which equalled to an average polymorphism rate of 7.2%.The size of amplified PCR products ranged from 50-400 bp.At a threshold of 0.51,these accessions were generally clustered into two groups.Genetic distance between single-cropping and double-cropping water bamboo was greater than the distance within each type,while the accessions with closer geographic origins showed higher genetic similarity.