内蒙古绒山羊孤核受体RORα部分cDNA克隆及序列分析

CLONING AND SEQUENCE ANALYSIS OF ROR α GENE IN INNER MONGOLIAN CASHMERE GOAT

依据已发表的牛、狗、人、小鼠等哺乳动物孤核受体RORα基因序列设计跨内含子引物,以内蒙古绒山羊cD-NA为模板进行RT-PCR扩增,得到417bp的基因序列,将扩增产物进行克隆测序后与GeneBank数据库进行序列同源性比较分析:绒山羊RORαcDNA扩增序列与牛、狗、猪和小鼠的同源性分别为98.3%、95.4%、95.2%和87.8%;与牛、猪、人、马、鼠相比RORα基因氨基酸序列同源性非常高,说明所获得序列为内蒙古绒山羊RORαcDNA序列,而且各种哺乳动物的RORα基因保守性较强。

According to the nucleotide sequences which published in the cow,dog,human and mouse,the RORα primers were designed.the RORα amplified by RT-PCR in Inner Mongolian cashmere goat and gain the 417 bp fragment.The PCR products were purified and cloned.Then recombinant plasmids were sequenced.The homology of nucleotide sequence of RORα gene between goat,cow,dog,human and mouse by blasting with database of GenBank were 98.3%,95.4%,95.2% and 87.8%,respectively.The homology of amino acid of RORα gene between cow,pig,horse,human and mouse were very high.It shows that the nucleotide which we sequenced is the Inner Mongolian cashmere goat RORα gene,and the conservation of the RORα gene is better in some of mammalian.