猪链球菌细胞外蛋白因子Ef基因的克隆与原核表达

Cloning and Expression of the Extracellular Protein factor of Streptococcus suis

采用分子克隆手段,将Ef全长基因克隆至表达载体pGEX-6p-1的Ptac启动子下游,电击转入大肠杆菌E.cohi BL21(DE3)中表达。经PCR扩增和蛋白质凝胶电泳鉴定,表明该EF蛋白获得有效表达,为进一步构建猪链球菌Ⅱ生物工程疫苗提供前期载体。

By molecular cloning method, full-length gene Ef was cloned into the downstream of the Ptac promoter of expression vector pGEX-6p-1, electric shock transformed into E. cohi BL21 （DE3） expression. After PCR amplification, and protein gel electrophoresis, the EF protein was effectively expressed, providing further construction of bio-engineering vaccine of Streptococcus suis serotype 2 with an initial vector.