沙利度胺对大鼠重症急性胰腺炎T淋巴细胞表型的影响

Effects of Thalidomide on the Changes of Lymphocyte Subsets in Rats with Severe Acute Pancreatitis

目的探讨大鼠重症急性胰腺炎(SAP)T淋巴细胞表型的改变及沙利度胺(反应停)对其的影响。方法 54只SD大鼠随机分成模型组(S组)、干预组(T组)和假手术组(C组),每组18只,采用5%牛磺胆酸钠胰胆管逆行注射方法建立SAP动物模型,SAP建模1h后用沙利度胺200mg/kg灌胃作为T组,开腹翻动胰腺后随即关腹作为C组。各组分术后3、6、12h3个时间点,处死大鼠收集外周血,利用流式细胞术检测外周血CD4+、CD8+、CD4+CD25+及CD3+-ICOS+的T细胞水平变化,对胰腺行常规病理学检测并评分。结果 (1)S组和C组相比,CD4+T细胞在3、6h明显增高,12h明显降低(均P<0.05);CD8+T细胞在6、12h明显下降(均P<0.05);CD4+CD25+T细胞在3、6、12h明显下降(均P<0.05);CD3+-ICOS+T细胞在6、12h明显下降(均P<0.05)。(2)T组和S组相比,CD4+T细胞在6h明显降低,12h明显增高(均P<0.05);CD8+T细胞在12h明显增高(P<0.05);CD4+CD25+T细胞有增高趋势,但差异无统计学意义(P>0.05),CD3+-ICOS+T细胞在12h明显增加(P<0.05)。(3)在3个时间点时S组的胰腺病理评分均较C组明显增高,T组较S组明显降低(均P<0.05)。结论沙利度胺的干预具有改善SAP时免疫紊乱的作用。

Objective To investigate the changes of lymphocyte subsets in rats with severe acute pancreatitis（SAP） and evaluate the effectiveness of thalidomide.Methods Fifty four SD rats were randomly divided into the three groups：the SAP group（group S）,treatment group（group T） and the control group（group C）.In each group there were 18 rats.Each group was divided into three different time measuring points：3th,6th,12th hour respectively.The models of SAP were induced by retrograde injection of 5% sodium taurocholate into the bili-pancreatic duct.Group T was made by administering thalidomide（200 mg /kg orally） at 1 h after SAP model was made.Group C was made just by tipping the pancreas.The proportion of Treg and lymphocyte subsets among peripheral lymphocytes was determined by flow cytometry.Results（1） Compared with group C,the percentage of CD4 ＋ cell in the group S was higher significantly at the 3th,6th hour,and decreased significantly at 12 h（all P〈0. 05） ;the percentage of CD8 ＋ cell in the group S decreased significantly at the 6th,12th hour（all P〈0. 05） ;the percentage of CD4^ ＋ CD25 ^＋ cell in the group S decreased significantly at three time points（all P〈0. 05） ;the percentage of CD3^ ＋ -ICOS ＋ cell in the group S decreased significantly at 6 th,12th hour.（2） Compared with group S,the percentage of CD4^ ＋ cell in the group T decreased significantly at 6th hour,and was higher significantly at the 12th hour（all P〈0. 05） ;the percentage of CD8 ＋ cell in the T group was higher significantly at the 12th hour（P〈0. 05） ;the percentage of CD4 ＋ CD25 ＋ cells in the group T was higher at 12th hour,but there was no significant difference（P〉0. 05） ;the percentage of CD3 ＋ -ICOS ＋ cell in the T group was higher significantly at the 12th hour（P〈0. 05）.⑶The pathological scores of pancreatic tissue in the group S was increased significantly at three time points,compared with group C（all P〈0. 05） ;the pathological scores of pancreatic tissue in the group T decreased significantly at three time points,compared with the group S（all P〈0. 05）.Conclusion Thalidomide can improve the immunologic derangement in rates with severe acute pancreatitis.