绝经后女性重度血管性痴呆患者雌激素治疗效果与雌激素受体相关受体亚型表达的关系

Correlation of the Expression of Estrogen Receptor Related Receptors Subtypes and the Effects of Estrogen Therapy in Postmenopausal Women with Severe Vascular Dementia

目的探讨绝经后重度血管性痴呆(VD)患者雌激素治疗效果与雌激素受体相关受体(ERR)α、β、γ各亚型表达的关系。方法以雌激素治疗的80例绝经后重度VD患者(有效组40例,无效组40例)在施行雌激素治疗前后的血液样本作为研究对象,同年龄阶段的80名正常老年绝经女性作为对照组。用ELISA测定各组患者治疗前后的血清雌二醇浓度。用半定量RT-PCR法检测VD有效组和无效组患者ERRs家族各亚型的mRNA在雌激素治疗前后的表达;用Western blot印迹法检测ERRs各亚型蛋白在雌激素治疗前后的表达。结果 ERRα、β和γ的mRNA和蛋白在VD患者显示特殊的表达模式:ERRα在无效组低表达,在有效组高表达,但表达均明显低于正常对照组(P<0.05);ERRβ在有效组、无效组和正常对照组之间基本无变化;ERRγ在无效组高表达,在有效组低表达,正常对照组表达最低(P<0.05)。结论 ERRα/ERRγ比值的改变可能与血管性痴呆的发病、雌激素的疗效有密切关系,EERs将是雌激素治疗重度血管性痴呆的分子生物学基础。

Objective To explore the association of the expression of estrogen receptor related receptor（ERR） α,β and γ with estrogen treatment in severe vascular dementia（VD） postmenopausal.Methods Forty patients with severe VD to whom estrogen therapy was effective were taken as the effective group,and another 40 patients with severe VD to whom estrogen therapy failed were taken as the ineffective group and another 80 normal postmenopausal women as the control group.Reverse transcription polymerase chain reaction was used to detect the mRNA in various subtypes of ERR family and Western blot was applied to determine the protein expression of ERR.Results There was a unique expression pattern in the mRNA and protein expressions of ERRα,β and γ in the VD patients.ERRα mRNA and protein expression was lower in the ineffective group and higher in the effective group,but the levels of ERRα mRNA and protein both in ineffective group and effective group were lower that those in the control group.ERRβ showed no significant differences among the effective group,ineffective group and control group.ERRγ expression was higher in ineffective group and lower in the effective group and was the lowest expression in control group.Conclusion These findings suggest that the change of ERRα/ERRγ ratio may be related to the development of vascular dementia and the efficacy of estrogen therapy in severe VD patients.EERs might be the bio-molecular basis of the estrogen treatment in severe vascular dementia.