人肝癌组织GGT异常表达与逆转录巢式PCR分析

Abnormal Expression and RT-PCR Analysis of γ-Glutamyl Transpeptidase Genes in the Tissues of Human Hepatocelullar Carcinoma

为探讨肝癌组织中γ-谷氨酰转肽酶（GGT）的表达与改变，将自身配对的肝癌、癌周及远癌组织总RNA纯化，分析不同分子形式GGT表达，并以逆转录巢式PCR（RT－PCR）扩增GGT5’-末端非编码区基因。结果：肝癌、癌周和远癌组总RNA浓度呈明显的梯度升高趋势（P＜0．05）；肝癌组总GGT比活性和不同分子形式GGT均明显高于癌周和远癌组（P＜0．05）；RT－PCR扩增GGT基因片段，在肝癌、癌周和远癌组阳性率分别为95．8％、87．5％和79．2％。提示肝癌组织中GGT呈异常表达，GGT基因分析可望成为监测肝细胞癌变的灵敏方法。

To explore the situation of expression of yg1utamyl transpeptidase (GGT) genes in thetissues of hepatocellular carcinoma, The t0tal RNA were purifled and quantified, the different molecu-lar forms of GGT were analyzed, The 5'-non coding (5'-NC) regions of the GGT genes were amplifiedusing reverse transcription nest po1ymerase chain reaction (RT-PCR) in the self-paired samples of hepa-tocellular carcinoma, adjacent paracancerous and distal cancerous tissues. Total RNA quantitationshowed an increasing tendency from carcinoma to distal cancerous. The specific activities (U/g) of totalGGT and the amount of different molecular forms of GGT were significantly higher in carcinoma tissuesthan those in paracancerous and distal cancerous tissues (P<0.O5). The positive rates of GGT genefragment amplified by RT-PCR were 95. 8% in hepatocellular carcinoma, 87. 5% in paracancerous tis-sue, and 79. 2% in distal cancerous tissues- The present data suggest that GGT gene in the tissues ofhepatocellular carcinoma was overexpressed and the analysis of GGT gene fragments may play a r01e inmonitoring the ear1y events 0f hepatocellular carcinoma.