金樱子组织培养及植株再生研究

Research on Tissue Culture and Plant Regeneration of Rosa laevigata Michx.

目的对金樱子茎段进行离体培养研究,为金樱子人工培育种苗提供依据。方法用带腋芽的茎段作外植体,采用不同激素配比的MS培养基进行诱导培养。结果金樱子丛生芽诱导用MS+6-BA1.8mg·L-1+KT0.2mg·L-1+NAA0.2mg·L-1培养基较佳;芽继代增殖用MS+6-BA1.5mg·L-1+KT0.2mg·L-1+NAA0.2mg·L-1培养基,其增殖系数达12.0;诱导生根用1/4MS+NAA0.2mg·L-1+IBA0.2mg·L-1培养基,根多、粗壮,生根率达100%。结论本研究结果对人工培育金樱子种苗是可行的。

Objective To settle the problem of seedlings shortage for artificial cultivation,the rapid propagation of Rosa laevigata Michx.in vitro culture was studied.Methods The axial buds were used as the explants and cultured in MS media with different hormones.Results The optimum axillary buds induction medium was MS ＋6-BA 1.8 mg · L^-1 ＋ KT 0.2 mg · L^-1＋ NAA 0.2 mg · L^-1;the subculture medium for bud proliferation was MS ＋6-BA 1.5 mg · L^-1 ＋ KT 0.2 mg · L^-1 ＋ NAA 0.2 mg · L^-1,and the multiplication coefficient reached 12.0;1/4 MS ＋ NAA 0.2 mg · L^-1 ＋ IBA 0.2 mg · L^-1 was used as rooting medium,the rooting rate reached 100%,with many strong root.Conclusion In this study,tissue culture of Rosa laevigata Michx.could supply a large number and high quality seedlings for artificial cultivation.