贵州黔北凹叶厚朴HPLC指纹图谱研究

HPLC Fingerprints of Magnolia officinalis Rehd.et Wils. in Guizhou Province

目的建立黔北凹叶厚朴的HPLC指纹图谱分析方法,为控制和评价黔北凹叶厚朴的质量提供依据。方法采用Diamonsil C18(4.6mm×200mm)柱;流动相:乙腈-水,梯度洗脱,检测波长为294nm;以厚朴酚为参照物,对黔北10批不同产地厚朴进行指纹图谱分析。结果建立黔北厚朴的HPLC指纹图谱共有模式,标定了8个共有峰,10批厚朴的相似度在0.921～0.983。结论该方法简便、准确、重复性好。可专属性地研究厚朴的指纹图谱,为有效地控制黔北凹叶厚朴的内在质量提供科学依据。

Objective To establish the analytical method of HPLC fingerprints of Magnolia officinalis and offer the evidence for quality control and quality evaluation carbonized Magnolia officinalis accordingly.Methods Ten batches of Magnolia officinalis were measured by HPLC with Magnolol as a reference substance and the HPLC analysis was performed on a DiamonsilC18 column（4.6 mm×200 mm）,with Acetonitrile and water as mobile phase and detection wavelength was 294 nm.Results The common mode of the HPLC fingerprints was set up.There were 8 common peaks in the fingerprint of ten samples,and the similar degrees to the ten batches were between 0.921～0.983.Conclusion The method is simple,accurate and reproducible.And the quality of Magnolia officinalis can be controlled effectively by the HPLC fingerprint.