慢性复合应激对大鼠皮质酮、血清代谢产物、免疫反应的影响及五味子的干预作用

Effects of Chronic Combined Stress on Corticosterone,Plasma Metabolites,and Immune Responsiveness in Rats and Intervention Effects of Schisandra

目的探讨五味子对慢性复合应激大鼠皮质酮、血糖、甘油三酯、胆固醇水平及免疫反应的影响。方法选取SD大鼠,随机分为安静对照组、应激对照组和五味子组,实验结束后测定血清皮质酮(CORT)、血糖(BG)、甘油三酯(TG)、总胆固醇(TC)、白介素-1(IL-1)、白介素-2(IL-2)及白介素-6(IL-6)水平,同时采用免疫组化方法检测大鼠垂体神经肽Y(NPY)细胞,电子显微镜观察垂体超微结构的变化。结果五味子可显著降低实验大鼠CORT水平及血糖水平,且能升高TG、TC水平;对应激大鼠免疫系统影响表现为IL-1、IL-2水平显著降低,IL-6水平变化不明显。结论五味子可降低应激大鼠血清皮质酮和血糖,改善应激所致的代谢紊乱。

Objective To investigate the influence of Schisandra on the corticosterone（CORT）,blood glucose（BG）,triglyceride（TG）,total cholesterin（TC） and immune responsiveness in male rats underwent chronic combined stress.Methods Thirty-four sprague-dawley（SD） rats were randomly divided into quiet group,stress control group and schisndra group.Blood samples were obtained at the end of the experiment to measure the CORT,BG,TG,TC,interleukin-1（IL-1）,IL-2 and IL-6 levels.The expression of neuropeptide Y cells in pituitary gland was detected by immunohistochemical technology.Meanwhile we excised the pituitary gland to observe the change of ultrastructure under electron microscope.Results In schisndra group BG,CORT,IL-1,IL-2 levels decreased significantly and TG,TC levels increased compared to stress control group.However,no significant differences were found in serum IL-6 levels among the three groups.Conclusion Schisandra have the effects on adjusting serum levels of CORT and blood glucose and improve the metabolic disorders by stress.