激素非依赖性前列腺癌T淋巴细胞亚群及NK细胞水平分析

Analyze the level of T lymphocyte subsets and NK cells with androgen-independent prostate cancer

目的观察激素非依赖性前列腺癌患者的细胞免疫水平。方法激素非依赖性前列腺癌患者36例,以处于激素依赖期前列腺癌患者39例为对照组,分别抽取静脉血,按程序处理后采用流式细胞术检测患者外周血T淋巴细胞亚群及NK细胞水平。结果与对照组比较,激素非依赖性前列腺癌患者CD3+T、CD4+T及CD8+T水平均明显下降(P<0.05),CD(16+56)+N水平亦下降明显(P<0.05),而CD4+/CD8+T比值上升,但差异无统计学意义;多脏器转移患者CD3+T、CD4+T、CD8+T及CD(16+56)+N水平下降更明显,与其他组分别比较差异有统计学意义(P<0.05)。结论激素非依赖性前列腺癌患者T淋巴细胞亚群水平及NK细胞活性明显下降,监测激素非依赖性前列腺癌患者的细胞免疫水平有助于判断患者病情变化和指导治疗。

Objective To investigate cell immunity of androgen-independent prostate cancer（AIPC）.Methods 36 cases of AIPC patients and 39 cases of androgen-dependent prostate cancer（regarded as control group） whose blood samples were collected in this study,T lymphocyte subsets and natural killer cells（NK cells） levels were detected by flow cytometry method.Results Compared with the control group,CD3＋、CD4＋、CD8＋ levels of AIPC all decreased significantly,CD（16＋56）＋ also decreased obviously,and the ratio of CD4＋/CD8＋T increased but without significant difference.The CD3＋、CD4＋、CD8＋ and CD（16＋56）＋ N levels of these AIPC patients who had multiple organ metastasis decreased more obviously in this study.Conclusion s The levels of T lymphocyte subsets and NK cells in AIPC patients decrease obviously,so monitor their cell immunity is helpful to estimate the conditions of AIPC and to guide the treatment methods.