实验大鼠梗阻性黄疸形成中TNF-α和IL-6对肝脏的影响

Effects of TNF α and IL 6 levels on liver during the formation of experimental rat obstructive jaundice

目的：探讨梗阻性黄疸形成过程中，血清及肝组织ＴＮＦα和ＩＬ６ 的水平及其与肝损害的关系。方法：以结扎胆总管（ｌｉｇａｔｉｎｇ ｃｏ ｍ ｍ ｏｎ ｂｉｌｅ ｄｕｃｔ ， ＬＣＤ） 的大鼠为模型， 采用放射免疫法， 测定ＬＣＤ 后不同时间血清和肝组织中ＴＮＦ－ α和ＩＬ－ ６ 的含量。结果：ＬＣＤ 组血清ＴＮＦα和ＩＬ６ 的水平［ 分别为（１２８５ ．００ ±７２ ．２３） μｇ／ Ｌ 和（３０１ ．２５ ±４４ ．８６） μｇ／ Ｌ］ 明显高于假手术对照组［ 分别为（７９１ ．００ ±１６４） μｇ／ Ｌ 和（２３５ ．００ ±４７） μｇ／ Ｌ］ ，（ Ｐ ＜ ０ ．０１ 和Ｐ ＜０ ．０５） ，肝组织中ＴＮＦα和ＩＬ６ 的含量［ 分别为（２３５３ ．７５ ±６９５ ．４５）μｇ／１００ｇ 和（９７２ ．７５ ±１５４ ．６２）μｇ／１００ｇ］ 也明显高于对照组（ 均为＜ ３００ μｇ／１００ｇ ，Ｐ ＜ ０ ．０１） ；于ＬＣＤ 后１ ｄ ～３ ｄ 变化最为明显，第７ 天～１４ 天维持在一较高水平。二者与谷丙转氨酶（ ＡＬＴ） 的变化均呈显著的正相关（ 相关系数ｒ 分别为０ ．６３１ 和０ ．７８２） 。结论：ＴＮＦα和ＩＬ６ 均参与了肝脏的损伤。

Aim: To investigate serum and hepatic tissue TNF α and IL 6 levels and their relationship to liver damage during the formation of experimental obstructive jaundice in rat. Methods:The rat model was estabilished by ligating common bile duct(LCD).TNF α and IL 6 levels in serum and hepatic tissue at different phases were detected by radioimmunoassay.Results:Serum TNF α and IL 6 levels 〔(1285.00±72.23)μg/L and (301.25±44.86)μg/L, respectively〕in the 14 d group rats after LCD were increased significantly as compared with that in the control group 〔(791±164)μg/L and (235.00±47)μg/L , P < 0.01 and P < 0.05, respectively〕. Hepatic tissue TNF αand IL 6 levels 〔(2353.75±695.45) μg/100 g and (972.75±154.62) μg/100g〕, increased significantly (P < 0.01) in the 14 d group rats after LCD, Serum levels of the two cytokines reached peak values on 1 to 3d after LCD. TNF α and IL 6 levels were higher on 7 day after LCD. Both the concentration of cytokines and serum ALT were well correlated (r=0.631 and r=0.782).Conclusion: Serum and hepatic tissue TNF αand IL 6 were increased significantly in LCD rats, and were well correlated to ALT,suggesting that TNF αand IL 6 play an important role in liver damage due to biliary obstruction.