蝙蝠葛碱对豚鼠缺氧心脏释放去甲肾上腺素量的影响

Effect of dauricine on the release of norepinephrine in anoxic guinea pig hearts

为探讨蝙蝠葛碱（Ｄａｕ）对缺氧心脏去甲肾上腺素（ＮＥ）释放的影响，运用高效液相色谱－电化学法测定不同缺氧条件下豚鼠离体心脏流出液中ＮＥ含量．结果显示：Ｄａｕ组（ｎ＝１３）停灌时心脏ＮＥ释放显著低于对照组（ｎ＝１１，Ｐ＜０．０１）；在低氧灌注，低氧酸性灌注及低氧高钾灌注电刺激时，Ｄａｕ组（ｎ＝１２－１３）心脏ＮＥ释放明显低于对照组（ｎ＝１１－１２，Ｐ＜０．００１）．表明Ｄａｕ可抑制缺氧心脏ＮＥ的释放．

In study of the effect of dauricine(Dau) on norepinephrine release in anoxic hearts, high performance liquid chromatography(HPLC) with electrochemical detection was used to determine the outflow of cardiac norepinephrine(NE) from perfused guinea pig hearts under different anoxic conditions. The results showed: the cardiac NE release of Dau group(n=13) was significantly less than that of control group(n=11) during stopped perfusion period(P<0.01); the cardiac NE release of Dau group(n=12-13) was markedly less than that of control group(n=11-12) at hypoxia perfusion, hypoxia low pH perfusion and hypoxia high K + perfusion(P<0.001). The result indicated that Dau could inhibit NE release in ischemic hearts.