麦胚黄酮类提取物对人乳腺癌细胞BCap-37的体外抑制作用

Inhibition of human breast cancer cell line BCap 37 by flavonoid extract of wheat germ in vitro

应用ＭＴＴ法、克隆形成实验、３Ｈ－ＴｄＲ掺入实验测定了麦胚黄酮类提取物对人乳腺髓样癌细胞株ＢＣａｐ－３７的生长抑制作用，结果显示麦胚黄酮类提取物能明显抑制人乳腺髓样癌细胞株ＢＣａｐ－３７的生长、克隆形成和ＤＮＡ合成能力，均呈现明显的剂量－效应关系，并随作用时间延长其效果增强；因此麦胚黄酮类提取物可能是通过抑制了ＢＣａｐ－３７细胞ＤＮＡ的合成而降低其生长和繁殖的能力。

Cell growth and proliferation were measured by microculture tetrazolium(MTT) assay,cell colony forming assay and the synthesis of DNA by 3H thymidine incorporation. Flavonoid extract of wheat germ resulted to a dose dependent,time dependent growth inhibition,reduction of colony and 3H thymiding incorporation in DNA of human breast cancer cell BCap 37.These findings indicated that the flavonoid extract of wheat germ can inhibit tumor cell growth and proliferation by blocking DNA synthesis in vitro.