体外长期培养人转化角朊细胞系的生物学特征

Biological characteristics of long term in vitro culture of SV40 transformed human keratinocyte line

目的：研究ＳＶ４０病毒转化的人角朊细胞系在体外长期培养过程中的细胞生物学特征．方法：采用细胞培养法观察转化细胞系的克隆形成率、细胞生命曲线以及血清、Ｃａ２＋对其生长分化的影响．结果：与正常角朊细胞相比，转化角朊细胞系于体外形成克隆所需的最低接种密度有所降低，但接触抑制特性仍未丧失．转化细胞在体外已传代培养２５代，对血清的依赖性降低，可被Ｃａ２＋诱导分化而形成细胞膜片结构．结论：本研究所观察的转化细胞系可在体外长期传代培养，并保留了正常角朊细胞的部分生物学特征，该细胞系在创面修复研究中具有一定价值．

AIM: To study the cytobiological characteristics of a SV40 transformed human keratinocyte line in the process of long term in vitro culture. METHODS: With cell culture technique, colony formation rate and life span curve of the cell line were determined and analyzed and the effects of serum and Ca 2+ on transformant growth and differentiation were observed. RESULTS: Compared with normal keratinocytes, the minimal seeding density of the transformed keratinocyte line required for in vitro colony formation was lower, but the contact inhibition feature of the transformants was retained. The cell line was subcultured for 25 passages in culture and its serum dependence reduced. The transformants were induced by Ca 2+ to differentiate and to form a cell sheet structure. CONCLUSION: The transformed cell line with some retained biological features shared by normal keratinocytes is capable of being subcultured in vitro for a long time and is valuable in wound repair research.