环孢霉素A对培养的人角朊细胞主要组织相容性抗原表达的影响

Effect of cyclosporin A on MHC expression of cultured human keratinocyte

目的：研究环孢霉素Ａ对人皮肤角朊细胞的主要组织相容性抗原（ＭＨＣ）表达的影响．方法：采用γ－干扰素（ＩＮＦ－γ）刺激培养的人角朊细胞ＭＨＣ－Ⅰ／Ⅱ抗原表达的实验模型，研究了低剂量环孢霉素Ａ对角朊细胞ＭＨＣ－Ⅰ／Ⅱ抗原表达的影响．结果：０．１２５ｍｇ／Ｌ的环孢霉素Ａ对于ＩＮＦ－γ刺激的角朊细胞ＭＨＣ－Ⅰ类抗原（ＨＬＡ－ＡＢＣ抗原）表达的增强，具有抑制作用（Ｐ＜０．０１）；但是对于ＩＮＦ－γ刺激的角朊细胞ＭＨＣ－Ⅱ类抗原（ＨＬＡ－Ｄｒ，ＨＬＡ－Ｄｐ抗原）表达的增强，没有抑制作用．

AIM: To study the effect of cyclosporin A on the major histocompatibility complex(MHC) antigen expression of human skin keratinocytes. METHODS: The influence of low dose cyclsporin A on keratinocyte MHC Ⅰ/Ⅱ antigen expression was assessed with an experimental model in which the expression of cultured human keratinocyte MHC Ⅰ/Ⅱ antigen was induced by INF γ. RESULTS: 0.125 mg/L cyclsporin A inhibited INF γ induced expression of keratinocyte MHC Ⅰ antigen (HLA ABC antigen) whereas no inhibitory effect on INF γ induced expression of keratinocyte MHC Ⅱ antigen (HLA Dr, HLA Dp antigen) was observed. CONCLUSION: The mechanism underlying prolonged human allo graft survival by cyclsporin A is related to the inhibitory effect of cyclsporin A on the keratinocyte MHC I expression of donor skin.