深Ⅱ°烫伤早期大鼠空肠P物质阳性神经与血管通透性的相关性变化

Correlation of Sub P positive nerves and vascular permeability in jejuna of rats in early stage deep partial thickness skin burns

目的：研究深Ⅱ°烫伤后早期大鼠空肠Ｐ物质（ＳＰ）阳性神经变化与血管通透性变化，探讨其相关性．方法：运用免疫组化技术和改良伊文思蓝渗出法，分别观察和检测大鼠空肠ＳＰ阳性神经变化和血管通透性变化．结果：烫伤后１ｈ大鼠空肠ＳＰ阳性神经密度达到最高值，后明显下降，烫伤后４ｈ～８ｈ达到最低值，随后逐渐恢复；烫伤后１ｈ大鼠空肠血管通透性最高，后缓慢下降，烫伤后８ｈ～２４ｈ达到最低值，而后略有升高，烫伤后７２ｈ接近正常值．深Ⅱ°烫伤后早期大鼠空肠ＳＰ阳性神经变化与血管通透性变化明显相关．结论：Ｐ物质是引起烫伤后早期血管通透性升高的重要介质之一．

AIM: To investigate the correlation of the changes of Sub P (SP) positive nerves and vascular permeability in the jejuna of rats in the early stage deep partial thickness skin burns. METHODS: Immunohistochemistry and modified Evans blue extravasation methods were used to observe and determine the changes of SP positive nerves and vascular permeability in the jejuna of rats and the correlation analysis was made. RESULTS: The distributed density of SP positive nerves in the jejuna of rats reached the highest value 1 h after burn and then decreased significantly. Between 4 h￣8 h, it reduced to the lowest, then it restored slowly. 72 h after burn it restored approximately to the normal value. The vascular permeability in the jejuna reached the highest value 1 h after burn, then decreased gradually. 8 h￣24 h, it reduced to the lowest. Then it increased slightly. The changes of SP positive nerves and vascular permeability in the jejuna of rats in the early stage deep partial thickness skin burns were significantly correlative. CONCLUSION: Sub P is an important mediator inducing increased vascular permeability after burns.