PDGF-AB对增生性瘢痕成纤维细胞增殖的影响

PDGF AB effects on proliferation of hypertrophic scar fibroblasts

目的：探讨ＰＤＧＦ－ＡＢ促进伤口愈合的作用机理及其在瘢痕增生中的作用．方法：取体外培养的人正常皮肤及增生性瘢痕成纤维细胞（ｎｏｒｍａｌｓｋｉｎｆｉｂｒｏｂｌａｓｔ，ＮＳＦＢ；ｈｙｐｅｒ－ｔｒｏｐｈｉｃｓｃａｒｆｉｂｒｏｂｌａｓｔ，ＨＴＳＦＢ）测定生长曲线，用ＭＴＴ法观察ＰＤＧＦ－ＡＢ对其增殖影响的差异．结果：两种细胞生长曲线存在差异，ＰＤＧＦ－ＡＢ对两种成纤维细胞增殖均有明显刺激作用，且均呈剂量依赖性关系，但作用有差异．结论：ＰＤＧＦ－ＡＢ可能通过刺激成纤维细胞增殖促进伤口愈合。

AIM: To investigate the mechanism by which PDGF AB promotes wound healing and the effects of PDGF AB on scar forming. METHODS: The effects of PDGF AB on cell proliferati of in vitro cultured human normal skin and hypertrophic scar fibroblasts were studied by MTT test and the growth curves of both of them were measured. RESULTS: Some differences were observed in the growth curves between human normal skin and hypertrophic scar fibroblasts and PDGF AB could enhance cell proliferation, which was dose dependent. CONCLUSION: PDGF AB may promote wound healing by stimulating cell division and proliferation and may play an important role in scar forming.