HPLC法同时测定养血化瘀合剂中阿魏酸和甘草酸的含量

Simultaneous Determination of Ferulic Acid and Glycyrrhizic Acid in Yangxuehuayu Mixture by HPLC

目的:建立HPLC法同时测定养血化瘀合剂中阿魏酸、甘草酸含量的方法。方法:采用Diamonsil C_(18)(200 mm×4.6mm,5μm)色谱柱,以乙腈为流动相A,0.085%磷酸水溶液为流动相B,进行线性梯度洗脱,流速为1.0 ml·min^(-1),检测波长为245 nm,柱温为25℃。结果:阿魏酸在5.02～50.24μg·ml^(-1)(r=0.9.99 9)和甘草酸在2.46～24.62μg·ml^(-1)(r=0.999 9)的范围内浓度与峰面积呈良好的线性关系,阿魏酸和甘草酸的加样回收率分别为100.1%(n=9,RSD=1.57%)和99.8%(n=9,RSD=0.98%)。结论:本法操作简单、快速、重复性好,结果准确可靠,可用于养血化瘀合剂的质量控制。

Objective： To establish an HPLC method for the simultaneous determination of ferulic acid and glycyrrhizic acid in yangxuehuayu mixture. Method： The Diamonsil C18 （200 mm ×4. 6 mm,5 ℃m）column was used. The mobile phase was acetonitrile and 0. 085 % phosphoric acid with gradient elution. The flow rate was 1 ml. min -1. The wavelength was 245 nm. The temperature of column was 25 ℃. Result： The linear ranges of ferulic acid and glycyrrhizic acid were 5.02-50. 24 μg·ml-1 （ r = 0. 999 9 ） and 2.46- 24. 62 μg· ml - 1 （ r = 0. 999 9 ）. The average recoveries were 100. 1% （ n = 9, RSD = 1.57% ） and 99. 8% （ n = 9, RSD = 0. 98% ）, respectively. Conclusion： The method is simple, reliable, and reproducible, it can be used to control the quality of yangxuehuayu mixture.