一步法RT-PCR检测手足口病EV71、CA16病毒核酸

One-step RT-PCR in Detecting EV71 and CA16 Viral Nucleic Acid of Hand-foot-mouth Disease

目的检测手足口病病原核酸,了解濮阳市手足口病病因和不同标本检测状况。方法对濮阳市辖区内手足口病43例住院患者的咽拭子、疱疹液和血清样本进行一步法RT-PCR检测。结果 43例患者的72份标本中,病原核酸EV71阳性23份,阳性率为31.94%。其中,咽拭子阳性率为39.53%(17/43),疱疹液阳性率为66.67%(6/9),血清阳性率为0%(0/20),CoxA16未检出。结论濮阳市手足口病流行由肠道病毒EV71引起,不同标本中疱疹液和咽拭子病毒含量多。

Objective To detect the pathogenic nucleic acid and different samples detection status of hand-foot-mouth disease（HFMD）,understanded the reasons which caused the disease in Puyang.Method Clinical samples included throat swab,vesicle fluid and serum from 43 HFMD inpatients in Puyang region were detected by one-step RT-PCR method.Results Among the 72 samples of 43 patients,23 were positive for pathogenic nucleic acid EV71（31.94%）,39.53%（17/43） for throat swab,66.67%（6/9） for vesicle fluid,and 0%（0/20） for serum,and CoxA16 was not detected at all.Conclusions The HFMD happen in Puyang is caused by enterovirus EV71.The vesicle fluid and throat swab have more virus than serum in different specimens.