SPE-HPLC法测定氯碘羟喹乳膏的含量

Determination of Clioquinol in Clioquinol Cream by HPLC

目的:建立测定氯碘羟喹乳膏含量的HPLC法。方法:采用C18固相萃取小柱分离除去氯碘羟喹乳膏中的基质,高效液相色谱法测定氯碘羟喹的含量。色谱柱为Zorbax C8柱(4.6mm×150mm,5μm);流动相为乙二胺四醋酸二钠盐缓冲液(取乙二胺四醋酸二钠盐0.5g,加水400mL,振摇使溶解,加三乙胺4mL,用磷酸调节pH值至3.0)-甲醇(35∶65),检测波长为254nm,流速1.0mL·min-1。结果:氯碘羟喹进样量在3～60μg范围内呈良好的线性关系(r=0.99995),平均回收率为99.0%(n=9)。结论:与现行方法比较,该方法专属性强,适用于测定氯碘羟喹乳膏含量。

Objective：To establish a method for the determination of Clioquinol in Clioquinol Cream by HPLC. Methods： The sample was purified with C18 solid phase column and its content was determined with HPLC method. The Zorbax Cs column （4. 6 mm × 150 mm, 5 μm） was used and mobile phase was EDTA-2Na buffer solution （dissolving 0. 50g of EDTA-2Na in 400 mL of water, adding 4. 0 mL of triethylamine and mixing, using phosphoric acid to adjust pH value of 3. 0） - methanol （35：65）. The detection wavelength was at 254nm, and the flow rate was 1.0 mL· min^-1. Results： Within the range of 3 -60 μg, peak area and clioquinol injection quantity were in linear Correlation （r =0. 999 95） and the average recovery were 99. 0% （n =9）. Conclusion. In comparison to the previous methods, the new method was more specific and can control quality of product effectively.