HPLC法同时测定华佗膏中水杨酸苯甲酸的含量

Content Determination of Salicylic Acid and Benzoic Acid in Huatuo Ointment by HPLC

目的:建立高效液相色谱法同时测定华佗膏中水杨酸苯甲酸的含量。方法:采用Agilent Eclipse XDB-C18色谱柱(4.6mm×250mm,5μm),流动相为甲醇-0.05mol·L-1磷酸氢二钠溶液(用磷酸调pH至4.6)(52∶48),柱温:30℃,流速为1.0mL·min-1,二极管阵列检测器(DAD),检测波长240nm,以外标法峰面积定量。结果:水杨酸与苯甲酸、空白样品分离良好,线性范围水杨酸为20.77～519.2μg(r=1.0000);苯甲酸为41.89～1047.3μg(r=1.0000),样品溶液在24h内稳定,平均回收率水杨酸为100.3%,RSD(n=9)为0.77%;苯甲酸为100.4%,RSD(n=9)为0.76%。结论:该方法简便、快速,测定结果准确可靠,重现性好,可用于华佗膏的质量控制。

Objective： To Determine the content of salicylic acid and benzoic acid in Huatuo ointment by HPLC. Methods：Agilent Eclipse XDB-C18 column（4. 6 mm ×250 mm, 5 μm） was used with the mobile phase of a mixture of Methanol and 0. 05 mol · L^-1 KH2PO4 （50： 50）, the pH was adjusted to 4.0 with phosphoric acid ; the flow rate was 1.0 mL · min ^- 1 ; the column temperature was 3022 ; The detection wavelength ： 240 nm. Results ： the sample and host were separated well by HPLC. The linear response range was 20. 77 - 519. 2 μg （r = 1. 000 0） of salicylic acid and 41.89 - 1 047. 3 μg （r = 1. 000 0） of benzoic acid; The sample solution is steady within 24 h. The average recoveries of salicylic acid and benzoic acid were 100. 3% （RSD = 0. 77% ）and 100. 4 （RSD = 0. 76% ）, respectively. Conclusions：This method is simple , rapid , accurate and reliable, and can used for the quality control of Huatuo Ointment.