摘要
目的:建立高效液相色谱法同时测定华佗膏中水杨酸苯甲酸的含量。方法:采用Agilent Eclipse XDB-C18色谱柱(4.6mm×250mm,5μm),流动相为甲醇-0.05mol·L-1磷酸氢二钠溶液(用磷酸调pH至4.6)(52∶48),柱温:30℃,流速为1.0mL·min-1,二极管阵列检测器(DAD),检测波长240nm,以外标法峰面积定量。结果:水杨酸与苯甲酸、空白样品分离良好,线性范围水杨酸为20.77~519.2μg(r=1.0000);苯甲酸为41.89~1047.3μg(r=1.0000),样品溶液在24h内稳定,平均回收率水杨酸为100.3%,RSD(n=9)为0.77%;苯甲酸为100.4%,RSD(n=9)为0.76%。结论:该方法简便、快速,测定结果准确可靠,重现性好,可用于华佗膏的质量控制。
Objective: To Determine the content of salicylic acid and benzoic acid in Huatuo ointment by HPLC. Methods:Agilent Eclipse XDB-C18 column(4. 6 mm ×250 mm, 5 μm) was used with the mobile phase of a mixture of Methanol and 0. 05 mol · L^-1 KH2PO4 (50: 50), the pH was adjusted to 4.0 with phosphoric acid ; the flow rate was 1.0 mL · min ^- 1 ; the column temperature was 3022 ; The detection wavelength : 240 nm. Results : the sample and host were separated well by HPLC. The linear response range was 20. 77 - 519. 2 μg (r = 1. 000 0) of salicylic acid and 41.89 - 1 047. 3 μg (r = 1. 000 0) of benzoic acid; The sample solution is steady within 24 h. The average recoveries of salicylic acid and benzoic acid were 100. 3% (RSD = 0. 77% )and 100. 4 (RSD = 0. 76% ), respectively. Conclusions:This method is simple , rapid , accurate and reliable, and can used for the quality control of Huatuo Ointment.
出处
《中国药品标准》
CAS
2010年第5期375-377,共3页
Drug Standards of China
关键词
高效液相色谱法
华佗膏
水杨酸
苯甲酸
含量测定
HPLC, Huatuo Ointment , Salicylic Acid , Benzoic Acid, content determination