利多卡因分子印迹聚合物的制备及其在固相萃取中的应用

Synthesis of Lidocaine Molecularly Imprinted Polymers and Application on Solid Phase Extraction

以利多卡因(LD)分子印迹聚合物为研究体系,利用分子模拟计算功能单体与模板分子之间的相互作用能,并通过作用实验加以验证,确定衣康酸为最佳单体。以乙二醇二甲基丙烯酸酯为交联剂,N,N-二甲基甲酰胺(DMF)为溶剂,采用本体聚合法合成分子印迹聚合物(MIP);采用高效液相色谱检测。实验发现,聚合物具有较高的印迹指数和选择性;在乙腈加载、5%甲醇-乙腈淋洗、5%HCl-甲醇洗脱条件下,聚合物具有最佳印迹效果。通过渗漏实验考察聚合物键合容量。实验发现,在乙腈溶液中每100mg MIP与空白聚合物(NIP)可分别键合1.62和0.92mg LD分子;采用光度法测定牛血清中利多卡因的含量,其平均回收率达到84.6%。

Itaconic acid was selected as the best template for lidocaine molecularly imprinted polymer （ MIP） through computational investigation and template-monomer interaction experiment. The MIP was prepared using ethylene glycol dimethacrylate as crosslinker and dimethylformamide as porogen. High retention factor and selectivity were achieved by HPLC evaluation of MIPs as column sorbent. After optimization of SPE profile,it was found that the best imprinting effect was obtained with loading in acetonitrile,washing with methanol-acetonitrile （ 5∶95,V/V） ,eluting with hydrochloric acid-methanol （ 5∶ 95,V/V） . MIP and nonimprinted polymer （ NIP） showed surprisingly high binding capacity in acetonitrile in breakthrough curve,and their capacities are 1. 62 and 0. 92 mg of drug to 100 mg of adsorbent respectively. Concentration of lidocaine in spiked serum was determined by visible spectrophotometric measurement of the SPE eluent,with the average recovery of 84. 6% .