Genetic Diversity Analysis and Constructing Core Collection Based on Phenotypes in Cotton

基于表型的棉花遗传多样性分析及核心材料的筛选(英文)

In that DNA diversity detected nowadays could not mean phenotypic diversity,it is the precondition of breeding project and basic research of crop improvement that genetic diversity analysis and sampling of core collection by phenotypes.Phenotyping and statistic analysis on 9 traits of 92 accessions of cotton germplasm resource from three species（Gossypium hirsutum L.,Gossypium barbadence L.and Gossypium arboreum L.）were conducted.And the statistics（variation coefficient,proportion of special accessions and Shannon-Weaver information index）indicated that initial collection had abundant phenotypic diversity;software NTSYS-pc and the unweighted pair group method of arithmetic（UPGMA）were used for the cluster analysis on genetic similarity coefficient and genetic distance matrix,and the result showed that the genetic relationship among accessions was highly consistent with the pedigree;22 accessions of core collection were selected by software QGAStation,four statistics,such as variance difference percentage（VD%）,mean difference percentage（MD%）,coincidence rate（CR%）and variable rate（VR%）,showed that the genetic diversity of core collection was approximately equal to the initial collection.The results of genetic diversity analysis based on phenotypic data and sampling of core collection would provide reference for breeding projects and basic research.

通过对来自3个栽培种(陆地棉、海岛棉和亚洲棉)的92份棉花资源的9个性状的调查和分析,变异系数、特异材料百分比和Shannon-Weaver多样性值均表明该群体蕴含较丰富的遗传变异;利用软件NTSYS-pc,采用类平均法(UPGMA)对相似系数矩阵和遗传距离矩阵进行聚类分析,结果表明品种间的遗传关系与品种所属的种及系谱吻合性较高;利用软件QGAStation筛选出核心材料22份,方差差异百分率、均值差异百分率、极差符合率和变异系数变化率相关统计学指标显示核心材料组遗传多样性与原群体基本一致。基于表型的遗传多样性分析及核心材料的筛选将为后续的育种工作和基础研究提供重要的参考信息。