不同月经周期人子宫内膜细胞的体外培养特性及鉴定

Exploration of the culturing characteristics and identification through the different menstrual cycle of endometrial cells in vitro

目的通过原代培养人子宫内膜细胞,进而建立正常子宫内膜的体外模型,同时观察不同月经周期内膜细胞的培养特性。方法选择因子宫肌瘤而行子宫全切术的12例患者,取子宫内膜细胞进行分离、纯化和培养。并用免疫细胞化学法进行鉴定。结果 12例患者中10例培养成功,在不同月经周期取材的内膜标本其培养细胞生长的数量、质量、成活率及形态上无明显差异;免疫细胞化学法结果显示,间质细胞CK19染色阴性,Vimentin染色阳性,阳性率90%,阳性细胞胞质呈棕黄色;腺上皮细胞CK19染色阳性,Vimentin染色阴性,阳性率85%,阳性细胞胞质染成棕黄色。结论本实验成功对子宫内膜细胞进行了体外的分离培养及鉴定,为研究细胞的生长、分化和代谢以及性激素的作用机制提供了一个理想的实验模型,同时认为内膜细胞的各种培养特性不存在明显的月经周期性改变。

Objective The human endometrial cells were cultured primarily to establish the normal endometrial model in vitro,so the different menstrual cycle characteristics of endometrial cells can be observed.Methods 12 cases of patients with uterine fibroids and hysterectomy were chosen,and then endometrial cells were separated,purified and cultured.And immunocytochemical identification method was used to identify it.Results 10 cases of 12 were cultured successfully.There is no significant difference in its quantity,quality,survival rate and patterns when they are in the menstrual cycle.The results of immunocytochemical showed that mesenchymal cells were negative when stained with CK19,and vimentin stain was positive,with the positive rate of 90%,and positive cells showed brown. On the contrary,Gland epithelial cells were stained by CK19,with the color showing brown,and the positive rate was 85%.Conclusion The isolation and identification of endometrial cells were tested successfully in vitro,which provide an ideal experimental model for the study of cell growth,differentiation and metabolism of endometrial cells,as well as sex hormone mechanism.At the same time,the culturing characteristics do not indicate any cyclical changes in menstruation.