线粒体DNA突变与氨基糖甙类抗生素致聋的关系

The relation between mitochondrial DNA mutation and aminoglycoside antibioticsinduced deafness

目的探讨线粒体ＤＮＡ突变与氨基糖甙类抗生素致聋的关系。方法应用ＰＣＲ－ＢｓｍＡＩ酶切及ＰＣＲ产物直接银染测序，对４８例氨基糖甙类抗生素致聋散发患者外周血标本的线粒体ＤＮＡ进行分析。结果发现６例患者线粒体ＤＮＡ１２ＳｒＲＮＡ基因核苷酸第１５５５位点发生了Ａ→Ｇ突变。结论氨基糖甙类抗生素致聋有明显的个体差异，带有１５５５Ｇ的个体对氨基糖甙类抗生素的耳毒作用有高度易感性。

Objective To investigate the relation between mitochondrial DNA mutation and aminoglycoside antibioticsinduced deafness. Methods Mitochondrial DNA (mtDNA) of peripheral blood from 48 sporadic cases was analysed using PCRBsmAI digestion and PCRdirect DNA silver sequencing. Results Six of 48 sporadic patients had A to G mutation in 12S rRNA gene of mtDNA at nucleotide 1555.THZConclusion The results suggest that mtDNA 1555G mutation is related to the occurring of aminoglycoside antibioticsinduced deafness which can cause genetic susceptibility to aminoglycoside antibotics ototoxicity.