肝癌组织GSTM1基因的缺失突变

The mutation of deletion for glutathione Stransferase M1 gene in the tissue of hepatocellular carcinoma

目的探讨在肝癌发生的过程中谷胱甘肽Ｓ－转移酶Ｍ１基因（ｇｌｕｔａｔｈｉｏｎｅＳ－ｔｒａｎｓｆｅｒａｓｅ，ＧＳＴＭ１）基因是否发生缺失突变。方法应用ＰＣＲ技术检测４６对肝癌组织和癌周肝组织的ＧＳＴＭ１基因型。结果肝癌组织ＧＳＴＭ１空白基因型的频率为７８．２６％，癌周肝组织则为６５．２２％（Ｐ＜０．０５）。６例肝癌组织的ＧＳＴＭ１空白基因型由癌周肝组织的非空白基因型转变而成，占１３．０４％。根据Ｈａｒｄｙ－Ｗｅｉｎｂｅｒｇ定律，推出ＧＳＴＭ１基因的缺失突变率为３８．８９％。结论在肝癌发生的过程中，ＧＳＴＭ１基因发生了缺失突变。

Objective To study whether the mutation of deletion for glutathione Stransferase M1(GSTM1) gene occurred during the development of hepatocellular carcinoma(HCC). Methods The genotypes of GSTM1 of 46 pairs of HCC tissue and the noncancerous liver tissue were detected by polymerase chain reaction(PCR). Results The frequency of GSTM1 null genotype for HCC tissue was 78.26%, but 65.22% for the noncancerous liver tissue (P<0.05).The GSTM1 null genotypes of 6 HCC tissue were transformed from the nonnull genotypes of the noncancerous liver tissue. According to HardyWeinberg law, the rate of deletion mutation for GSTM1 gene was inferred to be 38.89%. Conclusion The results suggested that the mutation of deletion for GSTM1 gene had occurred during the development of HCC.