阿霉素诱导十二指肠闭锁的实验研究

Congenital Duodenal Atresia in Rat Model Induced by Adriamycin

目的制作阿霉素诱导大白鼠胎仔出现先天性十二指肠闭锁的模型。方法Ｗｉｓｔａｒ孕鼠１０只，于妊娠第６至第９天腹腔注射阿霉素１７５ｍｐ／ｋｇ，妊娠２０天剖宫取胎仔，做解剖、电镜及光镜观察。结果用药组获胎仔３２只，十二指肠闭锁２６例（８１．３％），其中腔内闭锁１５．４％，闭锁近远端由纤维或胰腺相连者占４６．２％，近远端游离者为３８．５％，十二指肠闭锁畸形均伴有胰腺发育异常，９例胰颈、胰体及胰尾缺如，１７例胰体和胰尾缺如，另外，镜下见部分胰腺腺泡呈发育不良改变。结论阿霉素诱导大鼠胎仔出现先天性十二指肠畸形和胰腺畸形是一种可靠的模型制作方法。

Objective To describe a reprducible fetal model of duedenal atresia. Methods Themated Pregnant rats were given 1. 75 rug/kg of Adriamycin intraperitoneally on days 6 through 9 of gestation, and the litters were recovered on day 20. The fetuses were dissected microscopically and studied histologically. The findings were compared with those of age-matched saline embryos. Results All saline embryos were normal, whereas 81. 7% (26/32) of Adriamycin ernbryoo had duodenal atresia. The intralumi-nal atresia was found in 15. 4% (4/26) of duedenal atresia embryos, gapped atresia with pancreatic tissuefilled in between in 46. 2% (12/26) and gapped atresia in 38. 5 % (10/26). A double duedenal atresia wasfound in one fetus. The malformation was anatomically identical to that of human human. Interestingly,all duedenal atresia embryco were saaociated with pancreatic agenesis, in 34. 6 % with the pancreatic neck,ho and tail absence and in 65. 4% with the body and tail absence. Conclusion This easily repnducibleexperimental model permits new research into both its embryogenesis and the biology if the duedenal atresiafetus.