肿瘤坏死因子与丁硫氨酸亚砜胺协同抗卵巢癌作用的研究

The Synergistic Effect of Tumor Necrosis Factor andButhionone Sulfoximine on Ovarian Cancer

目的：研究肿瘤坏死因子（ＴＮＦ）和不同浓度的丁硫氨酸亚砜胺（ＢＳＯ）联合抗卵巢癌的作用。方法：用３ＡＯ、ＡＯ卵巢癌细胞株体外培养作为靶细胞，分别用ＴＮＦ、ＢＳＯ或联合作用于靶细胞，计算杀伤率。结果：单独应用ＴＮＦ和ＢＳＯ对３ＡＯ卵巢癌细胞无杀伤作用。用５００ｕ／ｍｌ的ＴＮＦ对ＡＯ卵巢癌细胞的杀伤率为３８４９％（Ｐ＜００５）。用５０ｕｇ／ｍｌＢＳＯ协同５０００ｕ／ｍｌＴＮＦ可杀伤３ＡＯ卵巢癌细胞４５９％（Ｐ＜００１）。对ＡＯ卵巢癌细胞的杀伤率为７５９％（Ｐ＜００１）。结论：联合应用明显优于单独应用。

Objective: To study synergistic effect of Tumor Necrosis Factor (TNF) Combined with different concentration Buthionone Sulfoximine (BSO) on ovarian cancer Methods: 3AO and AO Ovarian neoplasms cell lines were used as target in vito.TNFBSO or TNF combined with BSO was utilized respectively to test killer ratio. Resuls: If different concentration BSO and TNF were used respectively,3AO Ovarian cancer cells couldn't be killed.The killer ratio against AO Ovarian cancer cells was 3849% (P<005) with TNF(500/ml).The killer ratio against 3AO ovarian cancer cells was 459% (P<001) with TNF (5000/ml) plus BSO (50g/ml) and 759% (P<001) aginst AO. Conclution: Utilization of TNF combining with BSO is superior to that of respective TNF and BSO.