食管癌中P16、PCNA基因蛋白的表达及意义

P16 and PCNA Expression and Their Significance in Human Esophageal Squamous Cell Carcinoma

目的：探讨Ｐ１６、ＰＣＮＡ基因蛋白表达与食管鳞癌发生发展的关系。方法：应用Ｓ－Ｐ免疫组织化学方法对８４例食管鳞癌及１０例正常食管组织分别检测Ｐ１６蛋白、ＰＣＮＡ蛋白表达。结果：８４例食管鳞癌中Ｐ１６蛋白表达率为５４８％（４７／８４），Ｐ１６表达与淋巴结转移、临床分期、术后生存期显著相关（Ｐ＜００５）；ＰＣＮＡ表达阳性率与食管癌组织分化程度显著性正相关（Ｐ＜００５），淋巴结转移组显著高于未转移组，并与术后生存期互相关（Ｐ＜００１），与临床分期无显著相关。Ｐ１６与ＰＣＮＡ表达呈反相关系（Ｐ＜００１）。结论：表明Ｐ１６在食管癌生长、转移中起重要作用，Ｐ１６。

Objective:To investigate the possible relationship betwween P16 PCNA expression and their significance in human esophageal squamous cell carcinoma Methods:84 resected esophageal carcinomas speciments was examined for the expression of P16 PCNA proteins by using immunohistochemical S P method Results:The expression of P16 gene was 54 8%.The lower the differentiation of esophageal carcinoma,the lower the P16 positive rate ( P <0 01).The positive rate was significantly related to the ability of node metastasis TNM staging and postoperative ( P< 0 05).The PCNA positive cell rates of esophageal cancer were 12 9%、26 9%、and 47 5% in well、moderately and poorly differentiated carcinoma respectively and showed a significant negative correlation with survival the differentiation degree of the tumor and the ability of node metastasis( P< 0 001).Conclusion:the results suggest expression of P16 in esophageal carcinoma play an important role in suppression of invasive growth and metastasis P16 and PCNA may serve as a marker of predicting metastasis and prognosis of esophageal carcinoma.