SRRS方剂抑制消化道恶性肿瘤P^(53)基因突变的实验研究

The Effect of SRRS Prenventing P 53 Gene from Mutation

目的：从分子水平探讨ＳＲＲＳ方剂治疗消化道癌的可能机制。方法：选用Ｌｏｖｏ结肠腺癌细胞裸小鼠移植瘤模型，观察中药ＳＲＲＳ方剂抑制移植瘤生长的作用，并用ＰＣＲＳＳＣＰ银染色法研究ＳＲＲＳ方剂对Ｌｏｖｏ移植瘤生长过程中Ｐ５３基因突变的影响情况。结果：对照组有５０％（５／１０）发生Ｐ５３基因第七外显子突变，ＳＲＲＳ方剂治疗组无（０／１０）此现象（Ｐ＜００５）。结论：提示ＳＲＲＳ方剂能抑制Ｌｏｖｏ移植瘤生长过程中Ｐ５３基因的突变。

In order to study the mechanism of the tumor suppressor effect of the “SRRS”,a recipe composed of some Chinese herbs,the mutation of P 53 gene exon 7 in the tumor tissue was observed by PCR SSCP in the nude mice Lovo transplantation tumors.The important observation of this study was that none of the samples(0/10)was shown to have P 53 gene exon7 mutation in the experimental group which SRRS was taken.After all,50%(5/10) of the samples were shown to have P 53 mutation in the control group.This result suggested that SRRS could prevent P 53 gene from mutating during the Lovo transplatstion tumors growing.